^{Genomics and Molecular Biology}

Biography

Biography: Cedric Wu

Abstract

Engineering synthetic biological systems requires the design, build and testing of multi-gene networks. To optimize a synthetic system with the best combination of DNA parts, synthetic biologists will need to screen in a combinatorial design space.  But such combinatorial engineering approaches are hindered by the difficulty of simultaneously assembling numerous large, multi-gene constructs from a collection of DNA parts.

Here, we introduce our newly developed combinatorial library synthesis platform, which enables in-parallel construction of thousands of multiplexed gene variants in an efficient and economical way. This platform utilizes our patented semiconductor-based oligonucleotide microarray fabrication technology, high-throughput GenParts® DNA Fragments, and proprietary isothermal GenBuilderTM assembling reagents.  Libraries generated using this method are highly customizable and capable of generating over 1x108 variants.  As a result, our platform enables faster, easier, and more cost-effective engineering of metabolic pathways and microbial strains in comparison to alternative approaches.