Ahmed Abdelfatah
National Research Centre, Egypt
Title: Trigonelline attenuates hepatic complications and molecular alterations in high fat high fructose-induced insulin resistance in rats
Biography
Biography: Ahmed Abdelfatah
Abstract
The present study aimed to evaluate the effect of trigonelline (TRG) on the hepatic complications associated with high fat high fructose (HFHF)-induced insulin resistance (IR) in rats. IR was induced by adding saturated fat diet and 10% fructose in the drinking water to rats for 8 weeks. Insulin resistant rats were orally treated with TRIG (50 and 100 mg/kg), sitagliptin (SITA; 10 mg/kg) and the combination TRIG (50 mg/kg) with SITA (10 mg/kg) for 14 consecutive days. Afterwards, blood samples were withdrawn from fasting rats; liver and pancreas tissues were isolated. Sera were separated for determination of serum levels of glucose and insulin; to calculate the homeostatic model assessment-IR (HOMA-IR); and for determination of serum liver function. Liver homogenates were used for assessment of hepatic lipids, oxidative stress biomarkers and inflammatory cytokines. Histopathological and DNA cytometery examinations were carried out for hepatic and pancreatic tissues. Moreover, hepatic tissues were examined using Fourier Transform Infrared (FTIR) spectroscopy technique for assessment of any molecular changes. Results of the present study revealed that oral treatment of insulin resistant rats with TRG and its combination with SIT significantly decreased HOMA-IR, hepatic lipids, oxidative stress biomarkers; measured as malondialdehyde and reduced glutathione and the inflammatory cytokine; α-tumor necrosis factor. TRIG and its combination with SITA succeeded to ameliorate the histopathological, DNA cytometery and molecular alteration induced by HFHF. Finally, it can be concluded that TRIG has beneficial effects on the hepatic complications associated with HFHF-induced insulin resistance in rats due to its hypoglycemic effect and antioxidant potential.