John M Rosenfeld
EMD-Millipore, USA
Title: Genomics and chromatin analysis methods for profiling protein, RNA and DNA interactions in chromatin
Biography
Biography: John M Rosenfeld
Abstract
Chromatin immune-precipitation provides an in vivo picture of protein association within the dynamic cellular chromatin environment. Over the past decade, additional resolution on chromatin structure has been elucidated using other techniques that capture proteins or nucleic acids to uncover the composition of the components of chromatin, including regulatory proteins, modifi ed histones, modifi ed genomic DNA and the new putative chromatin regulatory molecule, non-coding RNAs. Th ese precipitation techniques, both immune based as well as nucleic acid capture based, allow dissection of the role of these molecules in establishing and characterizing chromatin state. Th e methods can be easily combined with current library construction techniques to provide genome wide views of these interactions under experimental treatments & genetic backgrounds. Th e information provided by ChIP (Chromatin Immuno-Precipitation), Nuclear RIP (RNA Binding Protein Immuno-Precipitation of chromatin) and ChIRP (Chromatin Isolation by RNA ) will be discussed.
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